This will allow quicker and easier gene knock-down experiments, for example, because having on-board Cas9 makes more room in vectors used to deliver the guide RNA to contain multiple instructions for ...

CRISPR-Cas9, a gene editing technology known for its user-friendliness, can knock genes in or out. Knocking out a gene involves inserting CRISPR-Cas9 into a cell using a guide RNA that targets the ...

该研究对用于 CRISPR 基因编辑治疗的 Cas 核酸酶 SaCas9 和 AsCas12a 进行了理性工程化改造，设计出了具有最小化免疫原性的 SaCas9 和 AsCas12a 突变体。

最近发表在开放获取期刊《基因组医学》（Genome Medicine）上的一项研究，报道了一种建立肝癌小鼠模型的新方法，即利用CRISPR ...

Highly efficient CRISPR knock-in in mouse Tokyo Medical and Dental University Journal Genome Biology Funder Strategic Research Program for Brain Sciences by MEXT, Tokyo Medical and Dental ...

GenScript now provides high-quality, sequence-verified HDR templates to help users edit their CRISPR experiments more ... levels of toxicity and higher knock-in efficiency Comparing GenWandTM ...

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in lymphoma development and therapy.

Gene knockout models involve the deliberate inactivation ... regularly interspaced short palindromic repeats ...

It’s a new way to create “bi-paternal” mice that can survive to adulthood—but human applications are still a long way off.

Advances in CRISPR technologies promise to accelerate the burgeoning pipeline of gene-editing therapies and broaden access to these disease-altering therapies.