Digital PCR, first described in 1997 by Olga Kalinina ... who developed a method called BEAMing, “And what BEAMing does is basically provide millions of wells in a single test tube.” ...

Overlay PCR reaction with 15 μl of mineral oil to prevent evaporation during the temperature cycling. Other DNA polymerases can also be used for this preamplification, depending on the fidelity ...