Probes then specifically bind to AAV capsids. Oligos on these probes ligate together when in close proximity. ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 ...

researchers add a unique pair of small barcodes consisting of a few nucleotides to the AAV sequence. Each viral genome also contains two flanking inverted terminal repeats (ITR) that enclose the ...

Previous efforts to circumvent the problem of the size limitation imposed by the AAV vector have focused mainly on altering the candidate genes into ‘mini-expression cassettes’ suitable for ...